01464nas a2200253 4500008004100000022002500041245008800066210006900154260001600223300001100239490000700250520069400257100001900951700001500970700002000985700002101005700002401026700002001050700001901070700002001089700002101109700002701130856005301157 2023 eng d a2224-8854, 1021-358900aA checklist of the dacine fruit flies (Diptera, Tephritidae, Dacinae) of Mozambique0 achecklist of the dacine fruit flies Diptera Tephritidae Dacinae cAug-08-2023 ae155850 v313 a
Here we present a checklist of all records of dacine fruit flies (Diptera: Tephritidae: Dacinae) from Mozambique, based on verified specimen records in natural history collections and literature records. In total, 57 Dacinae species are recorded from Mozambique, of which only one is considered endemic. This relatively low species diversity compared to other African countries appears to be related to incomplete sampling. For each species the localities from which it was recorded are given (including geocoordinates), or a general distribution is provided. The checklist is discussed briefly in terms of species richness, endemism and geographic distribution.
1 aDe Meyer, Marc1 aBota, Luis1 aDaniel, Beatriz1 aMussumbi, Mirene1 aVandenbosch, Myriam1 aCanhanga, Laura1 aCambula, Elias1 aMansell, Mervyn1 aCugala, Domingos1 aVirgilio, Massimiliano uhttp://dx.doi.org/10.17159/2254-8854/2023/a1558502738nas a2200277 4500008004100000245014700041210006900188520183900257100001502096700001102111700001802122700001502140700001902155700002702174700001902201700001502220700003102235700001802266700002902284700001602313700001602329700001702345700002602362700001602388856005602404 2024 eng d00aMitochondrial phylogenomics reveal the evolutionary, speciation and biogeographical history of fruit flies (Diptera: Tephritidae) UNDER REVIEW0 aMitochondrial phylogenomics reveal the evolutionary speciation a3 aFruit flies (Diptera: Tephritidae) possess high diversity and globally wide distribution, including a range of important agricultural pests. However, the origin, phylogeny and divergence history remain poorly understood. We undertook the first comprehensive mitogenomic study covering phylogenetic reconstruction, divergence time estimation and historical biogeography analyses by analyzing 147 mitochondrial genomes representing three subfamilies, seven tribes, 11 genera and 82 species. The results recovered all the three subfamilies, seven tribes and 11 genera as monophyletic group. Within Dacini subfamily, our phylogeny strongly supported the sister group relationship of (Zeugodacus + Dacus) + Bactrocera, which further supported to raise Zeugodacus as generic level of Dacini. On the other hand, our molecular phylogenetic analyses did not align well with the currently recognized subgeneric designations within Ceratitis, Dacus and Zeugodacus. Some subgenera were recovered as polyphyletic or paraphyletic, implying the incongruence between morphological characters and mitogenomic data. Neither the taxa within the Ceratitis FARQ complex nor those within the Bactrocera dorsalis complex could be clearly distinguished as distinct species corresponding to the morphospecies by mitochondrial phylogenetic tree. Divergence time estimation and historical biogeography analyses indicated that the earliest common ancestor of the Tephritidae, originated in the Palearctic realm at 138.92-71.44 Ma, with the subsequent divergence into Dacinae + Trypetinae and Tephritinae at 115.19-67.45 Ma equally in the Palearctic realm. Dacini might have originated in Laurasia rather than Gondwana and diverged into Bactrocera and Dacus + Zeugodacus at 48.43-28.70 Ma after India plate docked with Laurasia.
1 aZhang, Yue1 aLi, Hu1 aFeng, Shiqian1 aQin, Yujia1 aDe Meyer, Marc1 aVirgilio, Massimiliano1 aSingh, Sandeep1 aJiang, Fan1 aPriscilla, Kawi, Anastasia1 aSusanto, Agus1 aMartinez-Sañudo, Isabel1 aWu, Jiajiao1 aBADJI, Kemo1 aFekrat, Lida1 aDavaasambuu, Undarmaa1 aLi, ZHIHONG uhttp://dx.doi.org/10.22541/au.170669169.95739579/v102684nas a2200289 4500008004100000022002500041245009000066210006900156260001600225300001200241490000700253520180600260100001902066700001902085700001902104700002102123700002202144700002502166700002102191700002102212700002502233700001902258700002002277700002702297700002502324856004502349 2023 eng d a1314-2828, 1314-283600aDeveloping the Protocol Infrastructure for DNA Sequencing Natural History Collections0 aDeveloping the Protocol Infrastructure for DNA Sequencing Natura cOct-27-2023 ae1023170 v113 aIntentionally preserved biological material in natural history collections represents a vast repository of biodiversity. Advances in laboratory and sequencing technologies have made these specimens increasingly accessible for genomic analyses, offering a window into the genetic past of species and often permitting access to information that can no longer be sampled in the wild. Due to their age, preparation and storage conditions, DNA retrieved from museum and herbarium specimens is often poor in yield, heavily fragmented and biochemically modified. This not only poses methodological challenges in recovering nucleotide sequences, but also makes such investigations susceptible to environmental and laboratory contamination. In this paper, we review the practical challenges associated with making the recovery of DNA sequence data from museum collections more routine. We first review key operational principles and issues to address, to guide the decision-making process and dialogue between researchers and curators about when and how to sample museum specimens for genomic analyses. We then outline the range of steps that can be taken to reduce the likelihood of contamination including laboratory set-ups, workflows and working practices. We finish by presenting a series of case studies, each focusing on protocol practicalities for the application of different mainstream methodologies to museum specimens including: (i) shotgun sequencing of insect mitogenomes, (ii) whole genome sequencing of insects, (iii) genome skimming to recover plant plastid genomes from herbarium specimens, (iv) target capture of multi-locus nuclear sequences from herbarium specimens, (v) RAD-sequencing of bird specimens and (vi) shotgun sequencing of ancient bovid bone samples.
1 aFerrari, Giada1 aEsselens, Lore1 aHart, Michelle1 aJanssens, Steven1 aKidner, Catherine1 aMascarello, Maurizio1 aPeñalba, Joshua1 aPezzini, Flávia1 avon Rintelen, Thomas1 aSonet, Gontran1 aVangestel, Carl1 aVirgilio, Massimiliano1 aHollingsworth, Peter uhttp://dx.doi.org/10.3897/bdj.11.e10231700749nas a2200205 4500008004100000022002500041245017500066210006900241260001600310490000700326100002200333700002200355700001800377700002100395700001800416700001900434700002300453700002700476856004000503 2022 eng d a2045-8827, 2045-882700aEating eggplants as a cucurbit feeder: Dietary shifts affect the gut microbiome of the melon fly Zeugodacus cucurbitae (Diptera, Tephritidae)0 aEating eggplants as a cucurbit feeder Dietary shifts affect the cAug-01-20220 v111 aHendrycks, Wouter1 aDelatte, Hélène1 aMoquet, Laura1 aBourtzis, Kostas1 aMullens, Nele1 aDe Meyer, Marc1 aBackeljau, Thierry1 aVirgilio, Massimiliano uhttp://dx.doi.org/10.1002/mbo3.130703752nas a2200217 4500008004100000022001400041245016000055210006900215260001600284490000700300520299800307100001403305700001903319700002003338700002703358700002003385700003603405700002803441700001603469856004903485 2022 eng d a1756-330500aLarge-scale and small-scale population genetic structure of the medically important gastropod species Bulinus truncatus (Gastropoda, Heterobranchia)0 aLargescale and smallscale population genetic structure of the me cSep-19-20220 v153 aAbstract Background Gastropod snails remain strongly understudied, despite their important role in transmitting parasitic diseases. Knowledge of their distribution and population dynamics increases our understanding of the processes driving disease transmission. We report the first study to use high-throughput sequencing (HTS) to elucidate the population genetic structure of the hermaphroditic snail Bulinus truncatus (Gastropoda, Heterobranchia) on a regional (17–150 km) and inter-regional (1000–5400 km) scale. This snail species acts as an intermediate host of Schistosoma haematobium and Schistosoma bovis, which cause human and animal schistosomiasis respectively. Methods Bulinus truncatus snails were collected in Senegal, Cameroon, Egypt and France and identified through DNA barcoding. A single-end genotyping-by-sequencing (GBS) library, comprising 87 snail specimens from the respective countries, was built and sequenced on an Illumina HiSeq 2000 platform. Reads were mapped against S. bovis and S. haematobium reference genomes to identify schistosome infections, and single nucleotide polymorphisms (SNPs) were scored using the Stacks pipeline. These SNPs were used to estimate genetic diversity, assess population structure and construct phylogenetic trees of B. truncatus. Results A total of 10,750 SNPs were scored and used in downstream analyses. The phylogenetic analysis identified five clades, each consisting of snails from a single country but with two distinct clades within Senegal. Genetic diversity was low in all populations, reflecting high selfing rates, but varied between locations due to habitat variability. Significant genetic differentiation and isolation by distance patterns were observed at both spatial scales, indicating that gene flow is not strong enough to counteract the effects of population bottlenecks, high selfing rates and genetic drift. Remarkably, the population genetic differentiation on a regional scale (i.e. within Senegal) was as large as that between populations on an inter-regional scale. The blind GBS technique was able to pick up parasite DNA in snail tissue, demonstrating the potential of HTS techniques to further elucidate the role of snail species in parasite transmission. Conclusions HTS techniques offer a valuable toolbox to further investigate the population genetic patterns of intermediate schistosome host snails and the role of snail species in parasite transmission. Graphical Abstract
1 aMaes, Tim1 aDe Corte, Zoë1 aVangestel, Carl1 aVirgilio, Massimiliano1 aSmitz, Nathalie1 aDjuikwo-Teukeng, Félicité, F.1 aPapadaki, Maria, Ioanna1 aHuyse, Tine uhttp://dx.doi.org/10.1186/s13071-022-05445-x00666nas a2200205 4500008004100000022002500041245009900066210006900165260001600234300001600250490000700266100001800273700001900291700002700310700001900337700002500356700001900381700001900400856004100419 2022 eng d a0307-6970, 1365-311300aSystematics of Afrotropical Eristalinae (Diptera: Syrphidae) using mitochondrial phylogenomics0 aSystematics of Afrotropical Eristalinae Diptera Syrphidae using cJan-11-2022 a315 – 3280 v471 aMullens, Nele1 aSonet, Gontran1 aVirgilio, Massimiliano1 aGoergen, Georg1 aJanssens, Steven, B.1 aDe Meyer, Marc1 aJordaens, Kurt uhttp://dx.doi.org/10.1111/syen.1253200651nas a2200169 4500008004100000245015200041210006900193260001600262490000700278100002100285700002700306700002000333700002100353700001900374700001800393856007000411 2020 eng d00aComparative Microbiomics of Tephritid Frugivorous Pests (Diptera: Tephritidae) From the Field: A Tale of High Variability Across and Within Species0 aComparative Microbiomics of Tephritid Frugivorous Pests Diptera cNov-08-20200 v111 aDe Cock, Maarten1 aVirgilio, Massimiliano1 aVandamme, Peter1 aBourtzis, Kostas1 aDe Meyer, Marc1 aWillems, Anne uhttps://www.frontiersin.org/article/10.3389/fmicb.2020.01890/full00676nas a2200193 4500008004100000022001300041245013800054210006900192260001600261300001400277490000800291100001800299700001900317700001500336700002700351700001600378700002100394856006700415 2020 eng d a0141813000aMitogenomic characterization and systematic placement of the Congo blind barb Caecobarbus geertsii (Cypriniformes: Cyprinidae)0 aMitogenomic characterization and systematic placement of the Con cJan-10-2020 a292 - 2980 v1611 aMullens, Nele1 aSonet, Gontran1 aDecru, Eva1 aVirgilio, Massimiliano1 aSnoeks, Jos1 aVreven, Emmanuel uhttps://linkinghub.elsevier.com/retrieve/pii/S014181302033414000671nas a2200181 4500008004100000245013900041210006900180260001600249490000700265100002100272700002700293700002000320700002500340700002100365700001800386700001900404856006600423 2019 eng d00aImpact of Sample Preservation and Manipulation on Insect Gut Microbiome Profiling. A Test Case With Fruit Flies (Diptera, Tephritidae)0 aImpact of Sample Preservation and Manipulation on Insect Gut Mic cJan-12-20200 v101 aDe Cock, Maarten1 aVirgilio, Massimiliano1 aVandamme, Peter1 aAugustinos, Antonios1 aBourtzis, Kostas1 aWillems, Anne1 aDe Meyer, Marc uhttps://www.frontiersin.org/article/10.3389/fmicb.2019.02833/00840nas a2200265 4500008004100000022001400041245011000055210006900165260001600234300001400250490000700264100001900271700002100290700001400311700002700325700002200352700002800374700001800402700002300420700001700443700001400460700001900474700001900493856006200512 2019 eng d a0831-279600aFirst mitochondrial genomes of five hoverfly species of the genus Eristalinus (Diptera: Syrphidae)0 aFirst mitochondrial genomes of five hoverfly species of the genu cAug-07-2019 a677 - 6870 v621 aSonet, Gontran1 aDe Smet, Yannick1 aTang, Min1 aVirgilio, Massimiliano1 aYoung, Andrew, D.1 aSkevington, Jeffrey, H.1 aMengual, Ximo1 aBackeljau, Thierry1 aLiu, Shanlin1 aZhou, Xin1 aDe Meyer, Marc1 aJordaens, Kurt uhttp://www.nrcresearchpress.com/doi/10.1139/gen-2019-000900981nas a2200253 4500008004100000245018700041210006900228260001600297300000600313490000700319100002600326700001900352700002800371700003000399700002900429700002100458700003700479700002700516700002000543700003100563700002800594700002800622856007700650 2019 eng d00aFirst genomic study on Lake Tanganyika sprat Stolothrissa tanganicae: a lack of population structure calls for integrated management of this important fisheries target species0 aFirst genomic study on Lake Tanganyika sprat iStolothrissa tanga cJan-12-2019 a60 v191 aDe Keyzer, Els, L. R.1 aDe Corte, Zoë1 aVan Steenberge, Maarten1 aRaeymaekers, Joost, A. M.1 aCalboli, Federico, C. F.1 aKmentová, Nikol1 aMulimbwa, Théophile, N’Sibula1 aVirgilio, Massimiliano1 aVangestel, Carl1 aMulungula, Pascal, Masilya1 aVolckaert, Filip, A. M.1 aVanhove, Maarten, P. M. uhttps://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-018-1325-800775nas a2200229 4500008004100000022001400041245014200055210006900197260001600266300001600282490000700298100001900305700001300324700002200337700002700359700001900386700002200405700002100427700001900448700002300467856005500490 2018 eng d a0044-843500aUsing next-generation sequencing to improve DNA barcoding: lessons from a small-scale study of wild bee species (Hymenoptera, Halictidae)0 aUsing nextgeneration sequencing to improve DNA barcoding lessons cJul-08-2020 a671 – 6850 v491 aSonet, Gontran1 aPauly, A1 aNagy, Zoltán, T.1 aVirgilio, Massimiliano1 aJordaens, Kurt1 aVan Houdt, Jeroen1 aWorms, Sebastian1 aDe Meyer, Marc1 aBackeljau, Thierry uhttp://link.springer.com/10.1007/s13592-018-0594-y00626nas a2200169 4500008004100000022001400041245013100055210006900186260001600255300001400271490000700285100002200292700002700314700001900341700002400360856007200384 2017 eng d a0066-417000aTephritid Integrative Taxonomy: Where We Are Now, with a Focus on the Resolution of Three Tropical Fruit Fly Species Complexes0 aTephritid Integrative Taxonomy Where We Are Now with a Focus on cJul-01-2019 a147 - 1640 v621 aSchutze, Mark, K.1 aVirgilio, Massimiliano1 aNorrbom, Allen1 aClarke, Anthony, R. uhttp://www.annualreviews.org/doi/10.1146/annurev-ento-031616-03551800607nas a2200133 4500008004100000245017900041210006900220260001600289100001900305700002200324700002500346700002700371856007500398 2016 eng d00aDescription of new Ceratitis species (Diptera: Tephritidae) from Africa, or how morphological and DNA data are complementary in discovering unknown species and matching sexes0 aDescription of new Ceratitis species Diptera Tephritidae from Af cFeb-09-20181 aDe Meyer, Marc1 aMwatawala, Maulid1 aCopeland, Robert, S.1 aVirgilio, Massimiliano uhttp://www.europeanjournaloftaxonomy.eu/index.php/ejt/article/view/35700592nas a2200169 4500008004100000022001400041245008700055210006900142260001600211490000700227100001800234700001900252700002400271700002700295700002800322856007200350 2015 eng d a0066-417000aTaxonomy, Ecology, and Management of Native and Exotic Fruit Fly Species in Africa0 aTaxonomy Ecology and Management of Native and Exotic Fruit Fly S cOct-09-20150 v611 aEkesi, Sunday1 aDe Meyer, Marc1 aMohamed, Samira, A.1 aVirgilio, Massimiliano1 aBorgemeister, Christian uhttp://www.annualreviews.org/doi/10.1146/annurev-ento-010715-02360300713nas a2200205 4500008004100000022001400041245014200055210006900197260001600266300001400282490000700296100002200303700001700325700002000342700002000362700002200382700002700404700001900431856005700450 2015 eng d a0248-129400aPreference of Zeugodacus cucurbitae (Coquillett) for three commercial fruit vegetable hosts in natural and semi natural conditions0 aPreference of iZeugodacus cucurbitaei Coquillett for three comme cJan-11-2015 a333 - 3390 v701 aMwatawala, Maulid1 aKudra, Abdul1 aMkiga, Abdullah1 aGodfrey, Editha1 aJeremiah, Sigalla1 aVirgilio, Massimiliano1 aDe Meyer, Marc uhttp://www.fruits-journal.org/10.1051/fruits/201503400706nas a2200205 4500008004100000022001400041245012600055210006900181260001600250300001400266490000800280100002700288700002200315700002700337700002400364700001900388700001900407700002200426856005200448 2015 eng d a1313-298900aPopulation structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae)0 aPopulation structure and cryptic genetic variation in the mango cFeb-11-2017 a525 - 5380 v5401 aVirgilio, Massimiliano1 aDelatte, Hélène1 aNzogela, Yasinta, Beda1 aSimiand, Christophe1 aQuilici, Serge1 aDe Meyer, Marc1 aMwatawala, Maulid uhttp://zookeys.pensoft.net/articles.php?id=622100704nas a2200193 4500008004100000022001400041245015600055210006900211260001600280300001400296490000800310100001900318700002200337700002200359700001900381700003100400700002700431856005200458 2015 eng d a1313-298900aA review of the current knowledge on Zeugodacus cucurbitae (Coquillett) (Diptera, Tephritidae) in Africa, with a list of species included in Zeugodacus0 areview of the current knowledge on Zeugodacus cucurbitae Coquill cFeb-11-2017 a539 - 5570 v5401 aDe Meyer, Marc1 aDelatte, Hélène1 aMwatawala, Maulid1 aQuilici, Serge1 aVayssieres, Jean-François1 aVirgilio, Massimiliano uhttp://zookeys.pensoft.net/articles.php?id=622500619nas a2200169 4500008004100000022001400041245014800055210006900203260001600272300001400288490000800302100002200310700002700332700001900359700001900378856005200397 2015 eng d a1313-298900aWing morphometrics as a possible tool for the diagnosis of the Ceratitis fasciventris, C. anonae, C. rosa complex (Diptera, Tephritidae)0 aWing morphometrics as a possible tool for the diagnosis of the i cFeb-11-2017 a489 - 5060 v5401 aVan Cann, Joannes1 aVirgilio, Massimiliano1 aJordaens, Kurt1 aDe Meyer, Marc uhttp://zookeys.pensoft.net/articles.php?id=622600645nas a2200193 4500008004100000245010300041210006900144260001600213300001300229490000700242100001900249700001900268700002700287700002300314700001900337700001900356700002400375856005200399 2015 eng d00aDNA barcoding to improve the taxonomy of the Afrotropical hoverflies (Insecta: Diptera: Syrphidae)0 aDNA barcoding to improve the taxonomy of the Afrotropical hoverf cApr-10-2016 ae01402640 v101 aJordaens, Kurt1 aGoergen, Georg1 aVirgilio, Massimiliano1 aBackeljau, Thierry1 aVokaer, Audrey1 aDe Meyer, Marc1 aCasiraghi, Maurizio uhttp://dx.plos.org/10.1371/journal.pone.014026400774nas a2200157 4500008004100000022001300041245013900054210006900193260001600262100002700278700001900305700002400324700001900348700001900367856023000386 2015 eng d a1055790300aHigher phylogeny of frugivorous flies (Diptera, Tephritidae, Dacini): Localised partition conflicts and a novel generic classification0 aHigher phylogeny of frugivorous flies Diptera Tephritidae Dacini cJan-02-20151 aVirgilio, Massimiliano1 aJordaens, Kurt1 aVerwimp, Christophe1 aWhite, Ian, M.1 aDe Meyer, Marc uhttp://linkinghub.elsevier.com/retrieve/pii/S1055790315000111http://api.elsevier.com/content/article/PII:S1055790315000111?httpAccept=text/xmlhttp://api.elsevier.com/content/article/PII:S1055790315000111?httpAccept=text/plain00634nas a2200157 4500008004100000022001400041245009700055210006900152260001600221300001300237490000800250100002700258700001500285700001900300856015700319 2014 eng d a1313-298900aA set of multi-entry identification keys to African frugivorous flies (Diptera, Tephritidae)0 aset of multientry identification keys to African frugivorous fli cDec-07-2015 a97 - 1080 v4281 aVirgilio, Massimiliano1 aWhite, Ian1 aDe Meyer, Marc uhttp://www.pensoft.net/journals/zookeys/article/7366/abstract/a-set-of-multi-entry-identification-keys-to-african-frugivorous-flies-diptera-tephritidae-00769nas a2200205 4500008004100000022001400041245009700055210006900152260001600221300001400237490000800251100001900259700001900278700002000297700001900317700001900336700002300355700002700378856015800405 2013 eng d a1313-298900aAdhoc: an R package to calculate ad hoc distance thresholds for DNA barcoding identification0 aAdhoc an R package to calculate ad hoc distance thresholds for D cJun-12-2015 a329 - 3360 v3651 aSonet, Gontran1 aJordaens, Kurt1 aNagy, Zoltan, T1 aBreman, Floris1 aDe Meyer, Marc1 aBackeljau, Thierry1 aVirgilio, Massimiliano uhttp://www.pensoft.net/journals/zookeys/article/6034/abstract/adhoc-an-r-package-to-calculate-ad-hoc-distance-thresholds-for-dna-barcoding-identification00560nas a2200157 4500008004100000245012600041210006900167260001600236300001600252490000700268100002700275700001600302700002300318700001900341856004200360 2010 eng d00aMacrogeographic population structuring in the cosmopolitan agricultural pest Bactrocera cucurbitae (Diptera: Tephritidae)0 aMacrogeographic population structuring in the cosmopolitan agric cJan-07-2010 a2713 - 27240 v191 aVirgilio, Massimiliano1 aDELATTE, H.1 aBackeljau, Thierry1 aDe Meyer, Marc uhttps://jemu.myspecies.info/node/445800699nas a2200181 4500008004100000022001400041245022600055210006900281260001600350300001200366490000600378100001500384700002700399700001500426700001500441700001900456856004200475 2013 eng d a1877-725200aIsolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae)0 aIsolation and characterisation of sixteen microsatellite markers cJan-03-2013 a31 - 340 v51 aDelatte, H1 aVirgilio, Massimiliano1 aSimiand, C1 aQuilici, S1 aDe Meyer, Marc uhttps://jemu.myspecies.info/node/442300731nas a2200181 4500008004100000022001300041245016300054210006900217260001100286300001400297490000700311100002100318700002700339700001400366700001700380700001900397856013300416 2011 eng d a1055790300aDiscrepancies between subgeneric classification and molecular phylogeny of Ceratitis (Diptera: Tephritidae), can the evolution of host use provide some clues?0 aDiscrepancies between subgeneric classification and molecular ph c8/2011 a259 - 2640 v601 aErbout, Nathalie1 aVirgilio, Massimiliano1 aLens, Luc1 aBarr, Norman1 aDe Meyer, Marc uhttps://jemu.myspecies.info/discrepancies-between-subgeneric-classification-and-molecular-phylogeny-ceratitis-diptera-tephritida00562nas a2200169 4500008004100000022001400041245006700055210006700122260000900189300000800198490000700206100002700213700002300240700001800263700001900281856009200300 2010 eng d a1471-210500aComparative performances of DNA barcoding across insect orders0 aComparative performances of DNA barcoding across insect orders c2010 a2060 v111 aVirgilio, Massimiliano1 aBackeljau, Thierry1 aNevado, Bruno1 aDe Meyer, Marc uhttps://jemu.myspecies.info/comparative-performances-dna-barcoding-across-insect-orders00672nas a2200169 4500008004100000245012100041210006900162260001100231300001100242490000600253100002700259700001900286700002200305700002300327700001900350856013300369 2012 eng d00aIdentifying Insects with Incomplete DNA Barcode Libraries, African Fruit Flies (Diptera: Tephritidae) as a Test Case0 aIdentifying Insects with Incomplete DNA Barcode Libraries Africa c2/2012 ae315810 v71 aVirgilio, Massimiliano1 aJordaens, Kurt1 aBreman, Floris, C1 aBackeljau, Thierry1 aDe Meyer, Marc uhttps://jemu.myspecies.info/identifying-insects-incomplete-dna-barcode-libraries-african-fruit-flies-diptera-tephritidae-test-ca