Little guide on sequencing methodologies applied at JEMU

Sanger sequencing

Sanger sequencing is a targeted sequencing technique for a small number of pre-selected genomic regions. It is exceptionally useful in smaller-scale projects, test phases, result validation and obtaining sequence data of long contiguous DNA fragments.

Applications: DNA-based species identification, DNA barcoding, ancient and archive DNA analysis, single and multi-locus-based phylogenetic analyses, etc.

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Targeted amplicon sequencing (or parallel sequencing of PCR amplicons)

Using Next-Gen, targeted amplicon sequencing obtains sequence data of a large numbers of targeted gene regions of known utility for a large number of samples.

Applications: DNA-based species identification, DNA barcoding, ancient and archive DNA analysis, single and multi-locus-based phylogenetic analyses, etc.

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Genome skimming

Genome skimming is a simple, shallow sequencing method enabling the collection of the most repeated DNA fragments of the genome (mitochondrial genomes and ribosomal DNA) and is highly suitable for processing large batches of samples.

Applications: DNA-based species identification, DNA barcoding, multi-locus-based phylogenetic analyses, DNA metagenomics, etc.

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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith