@article {6508, title = {Why is the molecular identification of the forensically important blowfly species Lucilia caesar and L. illustris (family Calliphoridae) so problematic?}, volume = {223}, year = {2012}, month = {Nov-01-2012}, pages = {153-159}, issn = {0379-0738}, doi = {10.1016/j.forsciint.2012.08.020}, url = {http://dx.doi.org/10.1016/j.forsciint.2012.08.020}, author = {Sonet, Gontran and Jordaens, Kurt and Braet, Yves and Desmyter, Stijn} } @booklet {4476, title = {Conference volume. Program and abstracts{\textquoteright} of the Third European Conference for the Barcode of Life (ECBOL3), Barcoding of organisms of policy concern, Brussels, Belgium, 17-20 September}, year = {2012}, url = {http://ecbol3.myspecies.info/sites/ecbol3.myspecies.info/files/95815_Abstracts_ECBOL3.pdf}, author = {E.d. JEMU} } @article {4455, title = {Cold tolerance and freeze-induced glucose accumulation in three terrestrial slugs}, journal = {Comparative Biochemistry and Physiology Part A: Molecular \& Integrative Physiology}, volume = {161}, year = {2012}, month = {Jan-04-2012}, pages = {443 - 449}, issn = {10956433}, doi = {10.1016/j.cbpa.2012.01.002}, author = {Slotsbo, Stine and Hansen, Lars Monrad and Jordaens, Kurt and Backeljau, Thierry and Malmendal, Anders and Nielsen, Niels Chr. and Holmstrup, Martin} } @article {4437, title = {Taxonomic Identity of the Invasive Fruit Fly Pest, Bactrocera invadens: Concordance in Morphometry and DNA Barcoding}, journal = {PLoS ONE}, volume = {7}, year = {2012}, month = {May-09-2013}, pages = {e44862}, doi = {10.1371/journal.pone.0044862}, author = {Khamis, Fathiya M. and Masiga, Daniel K. and Mohamed, Samira A. and Salifu, Daisy and De Meyer, Marc and Ekesi, Sunday}, editor = {Filatov, Dmitry A.} } @article {4403, title = {First report of the exotic blue land planarian, Caenoplana coerulea (Platyhelminthes, Geoplanidae), on Menorca (Balearic Islands, Spain)}, journal = {ZooKeys}, volume = {199}, year = {2012}, month = {06/2012}, pages = {91}, issn = {1313-2989}, doi = {10.3897/zookeys.199.3215}, author = {Breugelmans, Karin and Quintana_Cardona, Josep and Artois, Tom and Jordaens, Kurt and Backeljau, Thierry} } @article {4400, title = {High species turnover of the ant genus Solenopsis (Hymenoptera : Formicidae) along an altitudinal gradient in the Ecuadorian Andes, indicated by a combined DNA sequencing and morphological approach}, journal = {Invertebrate Systematics}, volume = {26}, year = {2012}, month = {2012}, pages = {457-469}, issn = {1445-5226}, doi = {10.1071/IS12030}, author = {Delsinne, Thibaut and Sonet, Gontran and Nagy, Zolt{\'a}n T. and Wauters, Nina and Jacquemin, Justine and Leponce, Maurice} } @article {4396, title = {Identification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene}, journal = {International Journal of Legal Medicine}, volume = {127}, year = {2012}, month = {2012}, pages = {491-504}, issn = {1437-1596}, doi = {10.1007/s00414-012-0767-6}, author = {Jordaens, Kurt and Sonet, Gontran and Richet, Ren{\'e} and Dupont, Erena and Braet, Yves and Desmyter, Stijn} } @article {4395, title = {Why is the molecular identification of the forensically important blowfly species Lucilia caesar and L. illustris (family Calliphoridae) so problematic?}, journal = {Forensic Science International}, volume = {223}, year = {2012}, pages = {153-159}, issn = {03790738}, doi = {10.1016/j.forsciint.2012.08.020}, author = {Sonet, Gontran and Jordaens, Kurt and Braet, Yves and Desmyter, Stijn} } @article {4392, title = {Island Evolution and Systematic Revision of Comoran Snakes: Why and When Subspecies Still Make Sense}, journal = {PLoS ONE}, volume = {7}, year = {2012}, month = {8/2012}, pages = {e42970}, doi = {10.1371/journal.pone.0042970.t001}, author = {Hawlitschek, Oliver and Nagy, Zolt{\'a}n T. and Glaw, Frank}, editor = {O{\textquoteright}Grady, Patrick} } @inbook {53, title = {DNA Barcoding Amphibians and Reptiles}, booktitle = {DNA barcodes}, series = {Methods in Molecular Biology}, volume = {858}, year = {2012}, pages = {79 - 107}, chapter = {5}, issn = {978-1-61779-590-9}, author = {Vences, Miguel and Nagy, Zolt{\'a}n T. and Sonet, Gontran and Verheyen, Erik}, editor = {Kress, W John and Erickson, David L} } @article {4387, title = {Molecular Identification of Ceratitis capitata (Diptera: Tephritidae) using DNA Sequences of the COI Barcode Region}, journal = {Annals of the Entomological Society of America}, volume = {105}, year = {2012}, month = {03/2012}, pages = {339-350}, issn = {0013-8746}, doi = {10.1603/AN11100}, author = {Barr, N. B. and Islam, M. S. and DE MEYER, M. and McPheron, B. A.} } @article {4384, title = {Identifying Insects with Incomplete DNA Barcode Libraries, African Fruit Flies (Diptera: Tephritidae) as a Test Case}, journal = {PLoS ONE}, volume = {7}, year = {2012}, month = {2/2012}, pages = {e31581}, doi = {10.1371/journal.pone.0031581.t001}, author = {Virgilio, Massimiliano and Jordaens, Kurt and Breman, Floris C and Backeljau, Thierry and De Meyer, Marc} } @article {4382, title = {Host Shifts from Lamiales to Brassicaceae in the Sawfly Genus Athalia}, journal = {PLoS ONE}, volume = {7}, year = {2012}, month = {4/2012}, pages = {e33649}, doi = {10.1371/journal.pone.0033649.t003}, author = {Opitz, Sebastian E. W. and Boev{\'e}, Jean-Luc and Nagy, Zolt{\'a}n Tam{\'a}s and Sonet, Gontran and Koch, Frank and M{\"u}ller, Caroline} } @article {4381, title = {First Large-Scale DNA Barcoding Assessment of Reptiles in the Biodiversity Hotspot of Madagascar, Based on Newly Designed COI Primers}, journal = {PLoS ONE}, volume = {7}, year = {2012}, month = {3/2012}, pages = {e34506}, doi = {10.1371/journal.pone.0034506.t002}, url = {http://www.plosone.org/article/info\%3Adoi\%2F10.1371\%2Fjournal.pone.0034506}, author = {Nagy, Zolt{\'a}n T. and Sonet, Gontran and Glaw, Frank and Vences, Miguel} } @article {4378, title = {Analysis of tissue dependent DNA yield for optimal sampling of micro-moths in large-scale biodiversity surveys}, journal = {European Journal of Entomology}, volume = {109}, year = {2012}, pages = {1-6}, abstract = {DNA barcoding surveys of small insects usually extract DNA from either a complete insect or a leg. Little is known about how to optimize DNA quantity and quality from different insect parts while preserving a morphological voucher. Here, we quantify DNA yield from different body parts (antenna, hind leg, forewing, hind wing and abdomen) of the micro-moth Cameraria ohridella (Lepidoptera: Gracillariidae) using fluorescent nucleic acid stain (PicoGreen). Samples were preserved in 100\% ethanol or dried for three weeks. Our experiment was designed to encompass practical sampling options during fieldwork. DNA quality was assessed by PCR amplification of the mitochondrial COI barcode fragment. In addition, we compared PCR amplification using Platinum{\textregistered} Taq and Qiagen DNA Polymerase and quantified sequence success of amplified DNA. We show that overall, dry parts showed higher eluted DNA yields. PCR and sequencing success rate were slightly higher for dry tissue than ethanol-preserved parts. We also show that Platinum{\textregistered} Taq yielded the highest PCR success rate and that all dry tissues are sequenceable. The optimal strategy for DNA barcoding surveys is therefore to mount micro-Lepidoptera specimens in the field for morphological analysis and sample tissues (hind legs are favoured) from dried samples at a later time (several weeks) in the lab for DNA barcoding using preferentially Platinum{\textregistered} Taq. If larger amounts of DNA are required (i.e. for nuclear gene sequencing), several legs from one side of the specimen or the abdomen should be preserved in pure ethanol.}, keywords = {Cameraria ohridella, DNA barcoding, DNA preservation, DNA quantification, Gracillariidae, picogreen}, url = {http://www.eje.cz/scripts/viewabstract.php?abstract=1673}, author = {Lopez-Vaamonde, Carlos and Breman, Floris C and Lees, David, C and Van Houdt, Jeroen and De Prins, Jurate} }