- First time in our Laboratory? Please download the lab guidelines, fill it in and return it signed to the responsible of the lab. Thank you.
- Each lab user contributes to the organisation of the lab: see the list of lab jobs.
- Archival DNA from museum collection? Ask for a permission to use the archive DNA lab and read the guidelines that are specific to this lab.
- Sample tissue and keep track of everything in the lab book
- Working with different types of tissues? See specific recommendations
- Standard DNA extraction protocol using spin-columns: NucleoSpin® Tissue kit
- Booking sheet for the thermoshakers (form to be printed and completed in the pre-PCR lab)
- Example of standard protocol (25µl)
- Protocol for degraded DNA
- Primer database
- Booking sheet for the PCR machines (form to be printed and completed in the post-PCR lab)
- Spectrophotometry: NanoDrop TM 1000 spectrophotometer user manual
- Agarose gel electrophoresis
- Fluorometric quantitation
- Cycle sequencing: protocol of the manufacturer (BigDye® v.3.1.)
- Plate info (template) to import to sequencer
- Storing DNA using GenTegra (temporary protocol)
- Importing sample spreadsheets to the general database of the natural history collection: user manual and spreadsheet template.